{"product_id":"cst-3248s","title":"CST,  3248S, PKG-1 (C8A4) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying PKG1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, PKG-1 (C8A4) Rabbit Monoclonal Antibody (CST #3248) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPKG-1 (C8A4) Rabbit Monoclonal Antibody detects endogenous levels of total PKG-1 protein, including both α and β isoforms. This antibody does not cross-react with PKG-2.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to cental residues of human PKG-1.\n\u003cb\u003eBackground\u003c\/b\u003e\nCyclic GMP-dependent kinases (cGK\/PKG) belong to the AGC family of serine\/threonine protein kinases. In mammals, two genes encode PKG-1 and PKG-2. Alternative PKG-1 splicing yields α and β isoforms, which display tissue-specific expression patterns in humans (1). All PKG family members are activated by increased cellular cGMP, which binds to the enzyme's regulatory domain inducing a conformational change and leading to enzyme activation. cGMP levels are increased through the activation of guanylyl cyclases, a process known to occur in part through nitric oxide (NO) signaling (2). In addition to well established roles in platelet activation and smooth muscle relaxation (3), PKG signaling is important in many biological processes including cardiac contractility, axon guidance, bone growth, contraction of intestinal smooth muscle, and erectile dysfunction (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAAT8; cGK; cGK 1; cGK1; cGKI; cGKI-alpha; cGKI-BETA; cGMP-dependent protein kinase 1; cGMP-dependent protein kinase I; DKFZp686K042; FLJ36117; KGP1; MGC71944; PGK; PKG; PKG1; PRKG1; PRKG1B; PRKGR1A; PRKGR1B; protein kinase cGMP-dependent 1; protein kinase, cGMP-dependent, regulatory, type I, beta; protein kinase, cGMP-dependent, type I\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 78\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800975200425,"sku":"3248S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-3248s","provider":"Iright","version":"1.0","type":"link"}