{"product_id":"cst-32774s","title":"CST,  32774S, Aphidicolin","description":"Chemical Modulators for studying in the research area.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nAphidicolin is supplied as a lyophilized powder. For a 5 mM stock, reconstitute 1 mg of powder in 590 μl of DMSO. Working concentrations and length of treatment can vary depending on the desired effect.\n\u003cb\u003eStorage\u003c\/b\u003e\nStore lyophilized at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, store at -20ºC and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze\/thaw cycles.\n\u003cb\u003eBackground\u003c\/b\u003e\nAphidicolin is a tetracyclic diterpenoid first produced by the fungus . This antibiotic is a potent inhibitor of cellular deoxyribonucleic acid (DNA) synthesis by targeting α, ε, and δ DNA polymerases in eukaryotic cells lines with little to no effect on β or γ DNA polymerases (1,2). Studies have shown that Aphidicolin specifically binds to α DNA polymerase, resulting in the formation of a pol α-DNA-aphidicolin ternary complex that blocks DNA replication. The uses for this compound include synchronized cells at the G1\/S boundary and increased gene amplification (3-6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n(+)-Aphidicolin; (3R,4R,4aR,6aS,8R,9R,11aS,11bS)-Tetradecahydro-3,9-dihydroxy-4,11b-dimethyl-8,11a- methano-11aH-cyclohepta[a]naphthalene-4,9-dimethanol; (from Nigrospora sp.); ICI 69653; Nigrospora sp.; NSC 234714","brand":"CST","offers":[{"title":"Default Title","offer_id":46800694214825,"sku":"32774S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-32774s","provider":"Iright","version":"1.0","type":"link"}