{"product_id":"cst-32920s","title":"CST,  32920S, UBE3C (F2S2J) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying UBE3C. Validated for WB,WB,IP,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, UBE3C (F2S2J) Rabbit Monoclonal Antibody (CST #32920) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\nImmunoprecipitation: 1:100\nImmunofluorescence (Immunocytochemistry): 1:50 - 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nUBE3C (F2S2J) Rabbit Monoclonal Antibody recognizes endogenous levels of total UBE3C protein. Reactivity for immunofluorescence is human only.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly660 of human UBE3C protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nUbiquitin-Protein Ligase E3C (UBE3C) is an E3 ubiquitin-protein ligase that contributes to the regulation of multiple cellular processes through ubiquitination of various protein substrates, targeting them for proteasomal degradation. UBE3C activates the Wnt\/β-catenin pathway, promoting proliferation and inhibiting apoptosis in cancer cells (1,2). UBE3C-mediated protein degradation also regulates autophagy signaling (3,4), viral replication (5), type I interferon signaling (6), and radioresistance (7). In addition, UBE3C participates in eliminating misfolded protein aggregates by targeting misfolded proteins for degradation (8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nHECT-type ubiquitin transferase E3C; HectH2; KIAA0010; KIAA10; UBE3C; ubiquitin protein ligase E3C; ubiquitin-protein isopeptide ligase (E3); Ubiquitin-protein ligase E3C\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 123\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800979493033,"sku":"32920S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-32920s","provider":"Iright","version":"1.0","type":"link"}