{"product_id":"cst-3293s","title":"CST,  3293S, PHD-2\/Egln1 Antibody","description":"Polyclonal Antibody for studying PHD-2 (Egln1). Validated for Western Blotting. Highly specific and rigorously validated in-house, PHD-2\/Egln1 Antibody (CST #3293) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPHD-2\/Egln1 Antibody detects endogenous levels of total PHD-2\/Egln1 protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val226 of human PHD-2\/Egln1 protein. Antibodies are purified by peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nPHD-1 (Egln2), PHD-2 (Egln1), and PHD-3 (Egln3) are members of the Egln family of proline hydroxylases. They function as oxygen sensors that catalyze the hydroxylation of HIF on prolines 564 and 402, initiating the first step of HIF degradation through the VHL\/ubiquitin pathway (1,2). PHD-1 is highly expressed in a wide array of tissues, whereas PHD-2 and PHD-3 are expressed mainly in heart and skeletal muscle (1,3). The mRNA levels of PHD are upregulated by HIF through the hypoxia-response element under low oxygen conditions (4-7). These three enzymes also exhibit different peptide specificity target proteins, PHD-1 and PHD-2 can hydroxylate both proline 402 and proline 564, but PHD-3 can only hydroxylate proline 564 (2,8). In addition to HIF, PHD enzymes have also has been shown to catalyze the hydroxylation of RNA polymerase subunits and myogenin (3,9).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nC1orf12; DKFZp761F179; ECYT3; Egl nine homolog 1; egl nine homolog 1 (C. elegans); egl nine-like protein 1; egl-9 family hypoxia inducible factor 1; EGLN1; HALAH; HIF prolyl hydroxylase 2; HIF-PH2; HIF-prolyl hydroxylase 2; HIFPH2; HPH-2; HPH2; Hypoxia-inducible factor prolyl hydroxylase 2; PHD2; Prolyl hydroxylase domain-containing protein 2; SM-20; SM20; zinc finger MYND domain-containing protein 6; ZMYND6\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 50\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46800979689641,"sku":"3293S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-3293s","provider":"Iright","version":"1.0","type":"link"}