{"product_id":"cst-33056s","title":"CST,  33056S, b-Myb (F9W2M) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying B-Myb\/MYBL2. Validated for WB,WB,IP,ChIP. Available in 2 sizes. Highly specific and rigorously validated in-house, b-Myb (F9W2M) Rabbit Monoclonal Antibody (CST #33056) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits.\nWestern Blotting: 1:1000\nSimple Western™: 1:50 - 1:250\nImmunoprecipitation: 1:100\nChromatin IP: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Chromatin IP\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nb-Myb (F9W2M) Rabbit Monoclonal Antibody recognizes endogenous levels of total b-Myb protein. This antibody may detect a nonspecific band at 60 kDa.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human b-Myb protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nb-Myb, also known as MYBL2, is a member of the MYB transcription factor family and plays key regulatory roles in cell cycle, proliferation, and differentiation. b-Myb is essentially expressed in all proliferating cells, and b-Myb knockout mice are embryonically lethal (1,2). Importantly, b-Myb is overexpressed and reported to play a critical role in driving several cancers, including breast cancer, hepatocellular carcinoma, renal cell carcinoma, and non-small cell lung cancer (3-7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nB-Myb; BMYB; MGC15600; MYB proto-oncogene like 2; Myb-like protein 2; Myb-related protein B; MYBB; MYBL2; v-myb avian myeloblastosis viral oncogene homolog-like 2; v-myb myeloblastosis viral oncogene homolog (avian)-like 2; v-myb myeloblastosis viral oncogene homolog-like 2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 90\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800980213929,"sku":"33056S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-33056s","provider":"Iright","version":"1.0","type":"link"}