{"product_id":"cst-3322s","title":"CST,  3322S, Ras-GRF1 Antibody","description":"Polyclonal Antibody for studying RasGRF1 mouse. Validated for Western Blotting. Highly specific and rigorously validated in-house, Ras-GRF1 Antibody (CST #3322) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nRas-GRF1 Antibody detects transfected levels of Ras-GRF1. This antibody does not cross-react with Ras-GRF2.\nSpecies Reactivity: Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal residues of mouse Ras-GRF1. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nRas activity is regulated by GAP (GTPase activating proteins) and GEFs (guanine nucleotide exchange factors). Ras-GRF1 (also known as CDC25Mm) is neuronal RasGEF and is regulated by heterotrimeric G proteins and calcium influx (1,2). Binding to calmodulin and phosphorylation stimulate Ras-GRF1 activity (1,2). Multiple PKA phosphorylation sites on Ras-GRF have been identified. Phosphorylation on the two major sites, Ser54 and Ser822, inhibits Ras-GRF activity (3). Carbachol (a muscarinic agonist)-induced phosphorylation on Ser916 is essential but not sufficient for maximal Ras-GRF activity (4). It has been reported that Ras-GRF1 also shows GEF activity toward Rac after phosphorylation by the tyrosine kinase Src (5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAI844718; Cdc25; CDC25Mm; GNRP; Grf; GRF beta; Grf1; Grfbe; Grfbeta; guanine nucleotide releasing factor 1; Guanine nucleotide-releasing protein; p190; P190-A; p190RhoGEF; Ras guanine release factor beta; RAS protein-specific guanine nucleotide-releasing factor 1; Ras-GRF1; Ras-specific guanine nucleotide-releasing factor 1; Ras-specific nucleotide exchange factor CDC25; Rasgrf1; RGRF1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: M\nSENSITIVITY: Transfected Only\nMW (kDa): 155\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46800089514153,"sku":"3322S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-3322s","provider":"Iright","version":"1.0","type":"link"}