{"product_id":"cst-33386s","title":"CST,  33386S, ETS-2 (F7A6B) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Ets-2. Validated for WB,WB,IP,ChIP. Available in 2 sizes. Highly specific and rigorously validated in-house, ETS-2 (F7A6B) Rabbit Monoclonal Antibody (CST #33386) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits.\nWestern Blotting: 1:1000\nSimple Western™: 1:250\nImmunoprecipitation: 1:100\nChromatin IP: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Chromatin IP\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nETS-2 (F7A6B) Rabbit Monoclonal Antibody recognizes endogenous levels of total ETS-2 protein.\nSpecies Reactivity: Human, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human ETS-2 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nETS-2 is a member of the E26 Transformation-specific Sequence (ETS) transcription factor family, which binds to a core GGAA\/T DNA sequence in the promoter region of target genes to activate gene expression (1). ETS-2 activates expression of a wide variety of target genes, including s, , , , and oncogenic miRNAs (2-4). Its activity is important for cell growth and differentiation, bone formation, and autoimmune disease and cancer progression (5-7). Phosphorylation by MAP kinase at Thr72 upregulates the function of ETS-2, suggesting that therapeutic strategies targeting ETS-2 may provide an alternative to MEK inhibitor therapy (8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nETS proto-oncogene 2, transcription factor; Ets-2; ETS2; ETS2IT1; oncogene ETS-2; Protein C-ets-2; v-ets avian erythroblastosis virus E2 oncogene homolog 2; v-ets avian erythroblastosis virus E26 oncogene homolog 2; v-ets erythroblastosis virus E26 oncogene homolog 2; v-ets erythroblastosis virus E26 oncogene homolog 2 (avian)\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H Mk\nSENSITIVITY: Endogenous\nMW (kDa): 61\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800983687337,"sku":"33386S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-33386s","provider":"Iright","version":"1.0","type":"link"}