{"product_id":"cst-33593t","title":"CST,  33593T, ATF-3 (D2Y5W) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying ATF-3. Validated for Western Blotting,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, ATF-3 (D2Y5W) Rabbit Monoclonal Antibody (CST #33593) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits.\nWestern Blotting: 1:1000\nChromatin IP: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Chromatin IP\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nATF-3 (D2Y5W) Rabbit Monoclonal Antibody recognizes endogenous levels of total ATF-3 protein.\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant human ATF-3 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nActivating transcription factor 3 (ATF-3) is a basic leucine zipper-type transcription factor belonging to the ATF\/cAMP responsive element binding protein family. ATF-3 can form homodimers or heterodimers with other family members and depending on the cell types and promoter context, it can suppress or activate the transcription of its target genes (1,2). Expression of ATF-3 is induced by a variety of factors including cytokines, genotoxic agents, and physiological stress. For example, both metformin and high-density lipoprotein (HDL) can induce ATF-3 expression in macrophages (1,3-5). Research studies also indicate that ATF-3 can function as an oncogene or a tumor suppressor depending on the context (6,7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nactivating transcription factor 3; ATF-3; ATF3; cAMP-dependent transcription factor ATF-3; Cyclic AMP-dependent transcription factor ATF-3\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nMW (kDa): 23, 24\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800986144937,"sku":"33593T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-33593t","provider":"Iright","version":"1.0","type":"link"}