{"product_id":"cst-3363s","title":"CST,  3363S, Dicer Antibody","description":"Polyclonal Antibody for studying Dicer1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Dicer Antibody (CST #3363) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nDicer Antibody detects endogenous levels of total Dicer protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly1136 of human Dicer protein. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nDicer is a member of the RNase III family that specifically cleaves double-stranded RNAs to generate microRNAs (miRNAs) (1). After long primary transcript pri-miRNAs are processed to stem-looped pre-miRNAs by Drosha (2), pre-miRNAs are transported to the cytoplasm and further processed by Dicer to produce 22-nucleotide mature miRNAs (3). The mature miRNA then becomes a part of the RNA-Induced Silencing Complex (RISC) and can bind to the 3' UTR of the target mRNA (3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nDCR1; DICER; dicer 1, double-stranded RNA-specific endoribonuclease; dicer 1, ribonuclease III; dicer 1, ribonuclease type III; DICER1; Dicer1, Dcr-1 homolog; Dicer1e; Endoribonuclease Dicer; GLOW; Helicase MOI; Helicase with RNase motif; helicase-moi; HERNA; K12H4.8-LIKE; KIAA0928; MNG1; RMSE2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 220\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46800986538153,"sku":"3363S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-3363s","provider":"Iright","version":"1.0","type":"link"}