{"product_id":"cst-3419s","title":"CST,  3419S, Streptavidin (Sepharose® Bead Conjugate)","description":"Affinity and Purification Reagents for studying in the research area.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nAdd 10 μl of well-vortexed beads to 200 μl of cell lysate at 1 mg\/ml pre-incubated with biotinylated primary antibody. See protocol for more details.\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol. Store at -20°C. Do not aliquot the antibodies.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nStreptavidin has a remarkably high affinity for its natural ligand, biotin. The complex and irregular structure of the biotin-binding site makes it highly optimized for biotin binding and confers great specificity to the streptavidin-biotin complexes (4).\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nStreptavidin is expressed in .\n\u003cb\u003eBackground\u003c\/b\u003e\nStreptavidin is a 53,000 dalton tetrameric protein purified from the bacterium (3). Each subunit binds to biotin with extremely high affinity. Because of its strong non-covalent interaction with biotin, streptavidin can be used to isolate biotinylated proteins (1,2).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nBead Conjugate; Biotinylated; Streptavidin","brand":"CST","offers":[{"title":"Default Title","offer_id":46800798384297,"sku":"3419S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-3419s","provider":"Iright","version":"1.0","type":"link"}