{"product_id":"cst-34423s","title":"CST,  34423S, GOT1 (E4A4O) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying GOT1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, GOT1 (E4A4O) Rabbit Monoclonal Antibody (CST #34423) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nGOT1 (E4A4O) Rabbit Monoclonal Antibody recognizes endogenous levels of total GOT1 protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala344 of human GOT1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nGlutamate oxaloacetate transaminase 1 (GOT1) catalyzes the interconversion of aspartate and oxaloacetate (1). The increased transamination primarily catalyzed by GOT1 leads to elevated levels of 2-hydroxyglutarate, which promotes methylation of the gene locus, inhibits Foxp3 expression and activates T helper 17 (T 17) cell differentiation (2). In addition, GOT1 is critical to the survival of cells with electron transport chain inhibition by generating aspartate, a metabolite determining the proliferation of these cells (3-4). Studies also show that GOT1 plays a key role in the noncanonical glutamine pathway that supports liver tumorigenesis (5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAATC; aspartate aminotransferase 1; Aspartate aminotransferase, cytoplasmic; aspartate transaminase 1; AST1; ASTQTL1; cAspAT; cCAT; Cysteine aminotransferase, cytoplasmic; Cysteine transaminase, cytoplasmic; GIG18; Glutamate oxaloacetate transaminase 1; glutamic-oxaloacetic transaminase 1; glutamic-oxaloacetic transaminase 1, soluble; glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1); GOT1; growth-inhibiting protein 18; testis secretory sperm-binding protein Li 196a; Transaminase A\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 41\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799419343017,"sku":"34423S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-34423s","provider":"Iright","version":"1.0","type":"link"}