{"product_id":"cst-3464s","title":"CST,  3464S, Phospho-FLT3 (Tyr589\/591) (30D4) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying FLT3 (Tyr589\/Tyr591) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-FLT3 (Tyr589\/591) (30D4) Rabbit Monoclonal Antibody (CST #3464) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-FLT3 (Tyr589\/591) (30D4) Rabbit Monoclonal Antibody detects endogenous levels of FLT3 protein only when phosphorylated at tyrosine 589\/591. This antibody does not cross-react with other phosphorylated tyrosine kinases.\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to the sequences surrounding Tyr589\/591 of human FLT3.\n\u003cb\u003eBackground\u003c\/b\u003e\nFMS-related tyrosine kinase 3 (FLT3, also called FLK2) is a member of the Type III receptor tyrosine kinase family, which includes c-Kit, PDGFR, and M-CSF receptors. FLT3 is expressed on early hematopoietic progenitor cells and supports growth and differentiation within the hematopoietic system (1,2). FLT3 is activated after binding with its ligand FL, which results in a cascade of tyrosine autophosphorylation and tyrosine phosphorylation of downstream substrates (3). The p85 subunit of PI3 kinase, SHP2, GRB2, and Shc have all been reported to associate with FLT3 after FL stimulation (4-6). Tyr589\/591 is located in the juxtamembrane region of FLT3 and may play an important role in regulation of FLT3 tyrosine kinase activity. Somatic mutations of FLT3 consisting of internal tandem duplications (ITDs) occur in 20% of patients with acute myeloid leukemia (7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCD135; CD135 antigen; fetal liver kinase 2; Fetal liver kinase-2; FL cytokine receptor; FLK-2; FLK2; FLT-3; FLT3; FLT3 receptor tyrosine kinase; fms related receptor tyrosine kinase 3; fms related tyrosine kinase 3; Fms-like tyrosine kinase 3; fms-related tyrosine kinase 3; growth factor receptor tyrosine kinase type III; Receptor-type tyrosine-protein kinase FLT3; Stem cell tyrosine kinase 1; STK-1; STK1; Tyrosine-protein kinase receptor FLT3\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nMW (kDa): 160\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799421112489,"sku":"3464S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-3464s","provider":"Iright","version":"1.0","type":"link"}