{"product_id":"cst-34661s","title":"CST,  34661S, E2F8 (E8I3L) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying E2F8. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, E2F8 (E8I3L) Rabbit Monoclonal Antibody (CST #34661) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nImmunofluorescence (Immunocytochemistry): 1:800 - 1:3200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nE2F8 (E8I3L) Rabbit Monoclonal Antibody recognizes endogenous levels of total E2F8 protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human E2F8 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe E2F family consists of eight transcription factors that regulate gene expression to control cell cycle progression. The atypical E2F family members, E2F-7 and E2F-8, are unique in that they have two distinct DNA-binding domains, but lack the DP-heterodimerization, retinoblastoma-binding, and transcriptional activation domains contained in the canonical E2F family members. E2F-7 and E2F-8 are thought to function primarily as transcriptional repressors (1). E2F8 is highly expressed in multiple human cancers, including hepatocellular carcinoma, lung, breast, ovarian and cervical cancer, and may serve as a prognostic marker (1-5). In cancer cell lines, E2F8 expression enhances proliferation, migration, invasion, and the expression of epithelial mesenchymal transition (EMT) markers (2,3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nE2F family member 8; E2F transcription factor 8; E2F-8; E2F8; FLJ23311; Transcription factor E2F8\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 105\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799421407401,"sku":"34661S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-34661s","provider":"Iright","version":"1.0","type":"link"}