{"product_id":"cst-34859t","title":"CST,  34859T, mNeonGreen-Tag (E2S6M) Mouse Monoclonal Antibody","description":"Monoclonal Antibody for studying mNeonGreen lancelet. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, mNeonGreen-Tag (E2S6M) Mouse Monoclonal Antibody (CST #34859) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nmNeonGreen-Tag (E2S6M) Mouse Monoclonal Antibody detects recombinant proteins containing mNeonGreen protein. This antibody recognizes mNeonGreen protein fused to either the amino or carboxy terminus of targeted proteins in transfected cells. This antibody detects exogenously expressed mNeonGreen protein in cells expressed under a CMV promoter. Expression under other promoters has not been evaluated. This antibody does not cross-react with green-fluorescent protein (GFP). Non-specific signal of unknown origin may be observed in some rat cell lines.\nSpecies Reactivity: All Species Expected\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu18 of mNeonGreen protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nEpitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. mNeonGreen is a 220 amino acid, bright green-fluorescent protein derived from . mNeonGreen is a very useful tool as a fusion protein that reports gene expression, traces cell lineage, and defines subcellular protein localization (1).\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: All\nSENSITIVITY: Transfected Only\nSource\/Isotype: Mouse IgG1 kappa","brand":"CST","offers":[{"title":"Default Title","offer_id":46800214294697,"sku":"34859T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-34859t","provider":"Iright","version":"1.0","type":"link"}