{"product_id":"cst-34911t","title":"CST,  34911T, Phospho-Stat3 (Ser727) (D4X3C) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying STAT3 (Ser727) phosphate. Validated for WB,IP,IHC,IF,F,ChIP. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Stat3 (Ser727) (D4X3C) Rabbit Monoclonal Antibody (CST #34911) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits.\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:200\nImmunohistochemistry (Paraffin): 1:800 - 1:3200\nImmunofluorescence (Immunocytochemistry): 1:800\nFlow Cytometry (Fixed\/Permeabilized): 1:50\nChromatin IP: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 50804 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized), Chromatin IP\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-Stat3 (Ser727) (D4X3C) Rabbit Monoclonal Antibody is recognizes endogenous levels of Stat3 protein only when phosphorylated at Ser727.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic phospho-peptide corresponding to residues surrounding Ser727 of human Stat3 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Research studies have shown that Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAcute-phase response factor; ADMIO; ADMIO1; APRF; DNA-binding protein APRF; FLJ20882; HIES; MGC16063; Signal transducer and activator of transcription 3; signal transducer and activator of transcription 3 (acute-phase response factor); STAT3\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 86\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799423537321,"sku":"34911T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-34911t","provider":"Iright","version":"1.0","type":"link"}