{"product_id":"cst-3498t","title":"CST,  3498T, Bcl-2 (D17C4) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Bcl-2 (Ser70) phosphate mouse. Validated for Western Blotting,Immunoprecipitation. Available in 3 sizes. Highly specific and rigorously validated in-house, Bcl-2 (D17C4) Rabbit Monoclonal Antibody (CST #3498) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nBcl-2 (D17C4) Rabbit Monoclonal Antibody detects endogenous levels of total Bcl-2 protein.\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly41 of mouse Bcl-2.\n\u003cb\u003eBackground\u003c\/b\u003e\nBcl-2 exerts a survival function in response to a wide range of apoptotic stimuli through inhibition of mitochondrial cytochrome c release (1). It has been implicated in modulating mitochondrial calcium homeostasis and proton flux (2). Several phosphorylation sites have been identified within Bcl-2, including Thr56, Ser70, Thr74, and Ser87 (3). It has been suggested that these phosphorylation sites may be targets of the ASK1\/MKK7\/JNK1 pathway and that phosphorylation of Bcl-2 may be a marker for mitotic events (4,5). Mutation of Bcl-2 at Thr56 or Ser87 inhibits its anti-apoptotic activity during glucocorticoid-induced apoptosis of T lymphocytes (6). Interleukin-3 and JNK-induced Bcl-2 phosphorylation at Ser70 may be required for its enhanced anti-apoptotic functions (7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nApoptosis regulator Bcl-2; AW986256; B cell leukemia\/lymphoma 2; B-cell leukemia\/lymphoma 2; Bcl-; Bcl-2; Bcl2; C430015F12Rik; D630044D05Rik; D830018M01Rik; OTTMUSP00000024915\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nMW (kDa): 26\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799424422057,"sku":"3498T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-3498t","provider":"Iright","version":"1.0","type":"link"}