{"product_id":"cst-35293s","title":"CST,  35293S, Transferrin (E7F4T) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Transferrin. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Transferrin (E7F4T) Rabbit Monoclonal Antibody (CST #35293) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nTransferrin (E7F4T) Rabbit Monoclonal Antibody recognizes endogenous levels of total transferrin protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg663 of human transferrin protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nTransferrin is the major ferric iron transport protein, which binds ferric (Fe3+) ions in association with the binding of an anion, usually bicarbonate (1). The iron binding affinity of transferrin is pH dependent. In a neutral pH environment, transferrin (apotransferrin) binds iron with high affinity to form iron-bound transferrin (holotransferrin). In an acidic pH environment, the affinity of iron bound to transferrin decreases, dissociating iron from holotransferrin and releasing it into the environment (2). The importance of holotransferrin is to sequester Fe3+ ions in a relatively nonreactive and inert state to ensure normal free iron homeostasis in the body (3). Transferrin has been shown to contribute to iron-related oxidative damage (4), DNA synthesis (5), cell growth (6), and differentiation (7). Holotransferrin delivers iron to cells by binding to transferrin receptor (TFR1 or TFR2). Neutral pH at the cell surface promotes binding of holotransferrin to TFR1\/TFR2. The receptor-ligand complex enters the cell through receptor-mediated endocytosis and is internalized into an endosome. Relatively lower endosomal pH results in the release of iron. The receptor-ligand complex is recycled to the cell surface, where apotransferrin dissociates from TFR1\/TFR2 (8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nBeta-1 metal-binding globulin; DKFZp781D0156; epididymis secretory sperm binding protein Li 71p; HEL-S-71p; PRO1557; PRO2086; Serotransferrin; Siderophilin; TF; TFQTL1; Transferrin; TRFE\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 80\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799427141801,"sku":"35293S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-35293s","provider":"Iright","version":"1.0","type":"link"}