{"product_id":"cst-35509t","title":"CST,  35509T, LARS (D7Q4Q) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying LARS. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, LARS (D7Q4Q) Rabbit Monoclonal Antibody (CST #35509) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nLARS (D7Q4Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total LARS protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro788 of human LARS protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nLeucyl-tRNA Synthetase (LARS) is a leucine sensor critical for the activation of mTORC1 (1). mTORC1 kinase complex is an important component in the regulation of cell growth (2,3). Its activity is modulated by energy levels, growth factors, and amino acids (4,5). The four related GTPases, RagA, RagB, RagC, and RagD, have been shown to interact with raptor in mTORC1 (2,3). These interactions are both necessary and sufficient for mTORC1 activation in response to amino acid signals (2,3). LARS functions as a GTPase-activating protein (GAP) and interacts directly with RagD GTPase (1). The role of LARS in leucine sensing is not related to its tRNA charging activity (1).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\ncytoplasmic leucyl-tRNA synthetase; cytosolic leucyl-tRNA synthetase; FLJ10595; FLJ21788; hr025Cl; HSPC192; ILFS1; KIAA1352; LARS; LARS1; leucine translase; leucine tRNA ligase 1, cytoplasmic; Leucine--tRNA ligase; Leucine--tRNA ligase, cytoplasmic; leucine-tRNA ligase; leucyl-tRNA synthetase; leucyl-tRNA synthetase 1; Leucyl-tRNA synthetase, cytoplasmic; LeuRS; LEUS; LFIS; LRS; PIG44; proliferation-inducing gene 44; RNTLS; SYLC\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 134\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799429238953,"sku":"35509T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-35509t","provider":"Iright","version":"1.0","type":"link"}