{"product_id":"cst-35702sf","title":"CST,  35702SF, Insulin Receptor beta (A6H3G) Rabbit Monoclonal Antibody (BSA and Azide Free)","description":"Monoclonal Antibody for studying InsR-beta. Validated for Western Blotting,ELISA+. Highly specific and rigorously validated in-house, Insulin Receptor beta (A6H3G) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #35702) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nThis formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT\u0026amp;RUN or CUT\u0026amp;Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions\/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.\n\u003cb\u003eFormulation\u003c\/b\u003e\nSupplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free.\n\u003cb\u003eStorage\u003c\/b\u003e\nStore at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze\/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nInsulin Receptor beta (A6H3G) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of total insulin receptor β.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg1372 of human insulin receptor.\n\u003cb\u003eBackground\u003c\/b\u003e\nInsulin receptor (InsR) is a heterodimeric membrane receptor tyrosine kinase. It is composed of an extracellular α-subunit containing the ligand binding domain, a β-subunit containing an extracellular domain, a transmembrane domain, and a cytoplasmic tyrosine kinase domain (1). Binding of insulin to InsR results in receptor autophosphorylation and subsequent tyrosine kinase activation (2). This provides a docking site for various adaptor molecules, including insulin receptor substrate (IRS), Gab, and Shc, phosphorylation of which promotes subsequent activation of multiple downstream signaling pathways, including MAPK, PI3K, and TC10 (3,4). These events lead to increased glucose uptake and metabolism, and can promote cell growth. Loss-of-function mutation or desensitization of the InsR are two major contributors to insulin resistance and Type 2 diabetes (5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCD220; HHF5; INSR; Insulin receptor; Insulin receptor subunit alpha; Insulin receptor subunit beta; IR\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799431729321,"sku":"35702SF","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-35702sf","provider":"Iright","version":"1.0","type":"link"}