{"product_id":"cst-35980s","title":"CST,  35980S, FLI1 (D7N5M) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying FLI1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, FLI1 (D7N5M) Rabbit Monoclonal Antibody (CST #35980) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nFLI1 (D7N5M) Rabbit Monoclonal Antibody recognizes endogenous levels of total FLI1 protein.\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val64 of human FLI1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nFriend leukemia integration 1 (FLI1) transcription factor is an ETS domain-containing transcription factor that plays an important and highly conserved role in vertebrate development, particularly hematopoiesis, where it functions to activate transcription of genes that promote erythroblast proliferation (1-4). In mice, the locus is a common retroviral insertion site for the Friend murine leukemia virus (F-MuLV), such that a majority of F-MuLV-induced erythroleukemias are associated with aberrant Fli1 expression (5). Notably in humans, aberrant FLI1 expression has also been linked to poor prognosis in acute myeloid leukemia (6). Also in humans, a t(11;22)(q24;q12) chromosomal translocation has been described that generates a chimeric protein (EWS\/FLI1) comprised of the amino-terminal transactivation domain of Ewing's sarcoma breakpoint region 1 (EWS) and the carboxy-terminal ETS domain of FLI1 (7). The EWS\/FLI1 fusion protein functions as a transcriptional activator that is reportedly responsible for \u0026gt;85% of the known cases of pediatric Ewing's sarcoma, an aggressive bone and soft tissue tumor (8,9).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nBDPLT21; Ewing sarcoma breakpoint region 2; EWSR2; Fli-1 proto-oncogene, ETS transcription factor; FLI1; Friend leukemia integration 1 transcription factor; Friend leukemia virus integration 1; Proto-oncogene Fli-1; SIC-1; Transcription factor ERGB\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nMW (kDa): 52\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799438053545,"sku":"35980S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-35980s","provider":"Iright","version":"1.0","type":"link"}