{"product_id":"cst-3608s","title":"CST,  3608S, Notch1 (D1E11) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Notch1. Validated for WB,IP,IHC,ChIP. Available in 3 sizes. Highly specific and rigorously validated in-house, Notch1 (D1E11) Rabbit Monoclonal Antibody (CST #3608) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits.\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nImmunohistochemistry (Paraffin): 1:200 - 1:800\nChromatin IP: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 67334 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Chromatin IP\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nNotch1 (D1E11) Rabbit Monoclonal Antibody detects intracellular epitopes between 2400 and 2500 amino acids of human Notch1. This antibody recognizes both the full-length (~300 KDa) and the NTM region (~120 KDa), which consists of a short extracellular juxtamembrane peptide, a transmembrane sequence and the intracellular domain (NICD). This antibody antibody cannot detect the extracellular (ligand-binding) domain of Notch1 following cleavage at the S2 site by ADAM-type metalloproteases.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro2438 of human Notch1.\n\u003cb\u003eBackground\u003c\/b\u003e\nNotch proteins (Notch1-4) are a family of transmembrane receptors that play important roles in development and the determination of cell fate (1). Mature Notch receptors are processed and assembled as heterodimeric proteins, with each dimer composed of a large extracellular ligand-binding domain, a single-pass transmembrane domain, and a smaller cytoplasmic subunit (Notch intracellular domain, NICD) (2). Binding of Notch receptors to ligands of the Delta-Serrate-Lag2 (DSL) family triggers heterodimer dissociation, exposing the receptors to proteolytic cleavages; these result in release of the NICD, which translocates to the nucleus and activates transcription of downstream target genes (3,4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAOS5; AOVD1; hN1; Neurogenic locus notch homolog protein 1; NEXT; NICD; NOTC1; Notch 1; Notch 1 extracellular truncation; Notch 1 intracellular domain; Notch homolog 1, translocation-associated; Notch homolog 1, translocation-associated (Drosophila); notch receptor 1; NOTCH1; TAN1; Translocation-associated notch protein TAN-1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 120, 300\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799440609449,"sku":"3608S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-3608s","provider":"Iright","version":"1.0","type":"link"}