{"product_id":"cst-36135s","title":"CST,  36135S, POU2F3 (E5N2D) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying OCT11\/POU2F3. Validated for WB,IHC,IHC,ChIP,ChIP,C\u0026amp;R. Available in 2 sizes. Highly specific and rigorously validated in-house, POU2F3 (E5N2D) Rabbit Monoclonal Antibody (CST #36135) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT\u0026amp;RUN dilution was determined using CUT\u0026amp;RUN Assay Kit # 86652 .\nWestern Blotting: 1:1000\nIHC Leica Bond: 1:100 - 1:400\nImmunohistochemistry (Paraffin): 1:50 - 1:200\nChromatin IP: 1:50\nChromatin IP-seq: 1:50\nCUT\u0026amp;RUN: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 38561 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin), Chromatin IP, Chromatin IP-seq, CUT\u0026amp;RUN\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPOU2F3 (E5N2D) Rabbit Monoclonal Antibody recognizes endogenous levels of total POU2F3 protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu160 of human POU2F3 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nPOU2F3 (also known as OCT-11 and SKN-1) belongs to the OCT family of octamer motif-binding transcription factors, members of which play varied, diverse roles in animal development. POU2F3 is a lineage-defining transcription factor, responsible for driving differentiation of specialized chemosensory cells in the gastrointestinal and respiratory tracts (1-3). Tuft cells are a subtype of chemosensory cell, defined by POU2F3 expression, and which are hypothesized to be the origin of a distinct, non-neuroendocrine subtype of small cell lung cancer (SCLC) known as tuft cell-like SCLC (4). Consistent with its lineage-defining role in tuft cell development, POU2F3 was identified through a Crispr\/Cas9 screen as a master regulator of tuft cell-like SCLC (5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nEpoc-1; FLJ40063; MGC126698; Oct-11; OCT11; Octamer-binding protein 11; Octamer-binding transcription factor 11; OTF-11; OTF11; PLA-1; PLA1; PO2F3; POU class 2 homeobox 3; POU domain transcription factor OCT11a; POU domain, class 2, transcription factor 3; POU transcription factor; POU2F3; Skn-1a; Transcription factor PLA-1; Transcription factor Skn-1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 45-60\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799441297577,"sku":"36135S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-36135s","provider":"Iright","version":"1.0","type":"link"}