{"product_id":"cst-36177t","title":"CST,  36177T, Phospho-MFF Isoform 5 (Ser146) (F5U3I) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying MFF iso5 (Ser146) phosphate. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-MFF Isoform 5 (Ser146) (F5U3I) Rabbit Monoclonal Antibody (CST #36177) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-MFF Isoform 5 (Ser146) (F5U3I) Rabbit Monoclonal Antibody recognizes endogenous levels of MFF protein only when phosphorylated at Ser146. This antibody specifically detects isoform 5 of MFF protein (Q9GZY8-5).\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser146 of human MFF isoform 5 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nMitochondrial fission factor (MFF) is a tail-anchored protein that resides within the outer mitochondrial membrane and is part of the mitochondrial fission complex. MFF participates in mitochondrial fission by serving as one of multiple receptors for the GTPase dynamin-related protein 1 (Drp1) (1-4). Research studies have also shown that MFF is a peroxisomal membrane protein and participates in peroxisome fission by serving as a receptor for another GTPase, dynamin-like protein 1 (5,6). Research studies have demonstrated that the ability of MFF to drive acute mitochondrial fission in response to mitochondrial stress is controlled by AMPK-dependent phosphorylation. AMPK directly phosphorylates MFF at two sites, which allow for enhanced recruitment of Drp1 to the mitochondria (7). Multiple isoforms of MFF are expressed as a result of alternative splicing (8). One of the major MFF phosphorylation sites (Ser172 of human isoform 1\/Ser146 of human isoforms 2-5) lies within an AMPK phosphorylation motif that spans the boundary of differentially spliced MFF isoform exons, suggesting that AMPK phosphorylates MFF splice isoforms to varying degrees.\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nC2orf33; DKFZp666J168; EMPF2; GL004; MFF; MFF iso5; MGC110913; Mitochondrial fission factor\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 27\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799441723561,"sku":"36177T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-36177t","provider":"Iright","version":"1.0","type":"link"}