{"product_id":"cst-36425l","title":"CST,  36425L, Cleaved Gasdermin D (Asp275) (E7H9G) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying gasdermin D (Asp275) cleaved. Validated for Western Blotting,Immunoprecipitation,Immunohistochemistry (Paraffin). Available in 3 sizes. Highly specific and rigorously validated in-house, Cleaved Gasdermin D (Asp275) (E7H9G) Rabbit Monoclonal Antibody (CST #36425) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nImmunohistochemistry (Paraffin): 1:500 - 1:2000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier-free (BSA and azide free) version of this product see product # 37349 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCleaved Gasdermin D (Asp275) (E7H9G) Rabbit Monoclonal Antibody recognizes endogenous levels of the amino fragment of human gasdermin D protein only when cleaved at Asp275.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp275 of human Gasdermin D protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nGasdermin D (GSDMD), a member of the gasdermin family that includes GSDMA, GSDMB, and GSDMC, has been reported to have a critical role as a downstream effector of pyroptosis (1,2). Pyroptosis is a lytic type of cell death triggered by inflammasomes, multiprotein complexes assembled in response to pathogen-associated molecular patterns (PAMPs) or danger-associated molecular patterns (DAMPs) that result in the activation of caspase-1 and subsequent cleavage of pro-inflammatory cytokines IL-1β and IL-18 (3). Gasdermin D was identified by two independent groups as a substrate of inflammatory caspases, caspase-1 and caspase-11\/4\/5, producing two fragments: GSDMD-N and GSDMD-C. Cleavage results in release of an intramolecular inhibitory interaction between the N- and C-terminal domains, allowing the N-terminal fragment GSDMD-N to initiate pyroptosis through the formation of pores on the plasma membrane (4-7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nDF5L; DFNA5L; FKSG10; FLJ12150; gasdermin D; gasdermin domain containing 1; Gasdermin domain-containing protein 1; Gasdermin-D; Gasdermin-D, C-terminal; Gasdermin-D, N-terminal; GSDMD; GSDMD-CT; GSDMD-NT; GSDMDC1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 30\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799444082857,"sku":"36425L","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-36425l","provider":"Iright","version":"1.0","type":"link"}