{"product_id":"cst-36519t","title":"CST,  36519T, Phospho-HSP20 (Ser16) (E4W4X) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying HSP20\/HSPB6 (Ser16) phosphate. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-HSP20 (Ser16) (E4W4X) Rabbit Monoclonal Antibody (CST #36519) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-HSP20 (Ser16) (E4W4X) Rabbit Monoclonal Antibody recognizes endogenous levels of HSP20 protein only when phosphorylated at Ser16.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser16 of human HSP20 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe small heat shock protein 20 (HSP20\/HSPB6) is a ubiquitously expressed molecular chaperone, with higher levels found in skeletal, cardiac, and smooth muscle (1). HSP20 plays a cardioprotective role in response to injury through cardiac β-adrenergic signaling, which induces increased HSP20 protein expression and phosphorylation at Ser16 by PKA (2-4). Phosphorylation of HSP20 at Ser16 protects cells from undergoing apoptosis (5) and mediates HSP20 interaction with the cytoskeleton (6). Constitutive phosphorylation of HSP20 at Ser16 in mice leads to fibrosis and heart failure through interleukin-6 (IL-6) and STAT3 signaling (7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nepididymis luminal protein 55; epididymis secretory sperm binding protein; FLJ32389; Heat shock 20 kDa-like protein p20; Heat shock protein beta-6; heat shock protein family B (small) member 6; heat shock protein family B (small) member B6; heat shock protein, alpha-crystallin-related, B6; HEL55; Hsp20; HSPB6; PPP1R91; protein phosphatase 1, regulatory subunit 91\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 17\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799444770985,"sku":"36519T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-36519t","provider":"Iright","version":"1.0","type":"link"}