{"product_id":"cst-36788t","title":"CST,  36788T, GCH1 (F8T4M) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying GCH1. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, GCH1 (F8T4M) Rabbit Monoclonal Antibody (CST #36788) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunofluorescence (Immunocytochemistry): 1:1600 - 1:3200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nGCH1 (F8T4M) Rabbit Monoclonal Antibody recognizes endogenous levels of total GCH1 protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly52 of human GCH1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nGTP cyclohydrolase 1 (GCH1) catalyzes the rate-limiting step of tetrahydrobiopterin (BH ) biosynthesis. Studies show that BH functions as a radical-trapping antioxidant and protects cells against ferroptosis independently of GPX4. In addition, dihydrofolate reductase (DHFR) is required to maintain BH levels by regenerating the compound (1,2). Furthermore, deficiency of spliceosomal GTPase Eftud2 in mouse cerebellar Purkinje cells (PCs) causes severe ferroptosis, PC degeneration, dyskinesia, and cerebellar atrophy. Ablation of in PCs downregulates expression through mis-splicing of , activating ferroptosis (3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\ndystonia 14; DYT14; DYT5; DYT5a; GCH; GCH1; GTP cyclohydrolase 1; GTP cyclohydrolase I; GTP-CH-1; GTP-CH-I; GTPCH1; guanosine 5'-triphosphate cyclohydrolase I; HPABH4B\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 28\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799447261353,"sku":"36788T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-36788t","provider":"Iright","version":"1.0","type":"link"}