{"product_id":"cst-3691t","title":"CST,  3691T, Phospho-eEF2k (Ser366) Antibody","description":"Polyclonal Antibody for studying eEF2K (Ser366) phosphate. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-eEF2k (Ser366) Antibody (CST #3691) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-eEF2k (Ser366) Antibody detects endogenous levels of eEF2k only when phosphorylated at Ser366.\nSpecies Reactivity: Human, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser366 of human eEF2k. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nEukaryotic elongation factor 2 kinase (eEF2k) phosphorylates and inactivates eEF2, resulting in the inhibition of peptide-chain elongation (1). eEF2k is normally dependent on Ca2+ ions and calmodulin (2,3). It can be activated by PKA in response to elevated cAMP levels (4-6), which are generally increased in stress- or starvation-related conditions. eEF2k can also be regulated in response to a wide range of stimuli that promote cell growth and protein synthesis. This involves the phosphorylation of eEF2k by p90RSK and p70 S6 kinase at Ser366 or by SAPK4\/p38delta at Ser359, leading to the inactivation of eEF2k (7,8), which facilitates the dephosphorylation of eEF2, and thus promotes translation.\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nalternative protein EEF2K; Calcium\/calmodulin-dependent eukaryotic elongation factor 2 kinase; calcium\/calmodulin-dependent eukaryotic elongation factor-2 kinase; calmodulin-dependent protein kinase III; CaMKIII; eEF-2 kinase; eEF-2K; EEF2K; EF2K; elongation factor 2 kinase; elongation factor-2 kinase; eukaroytic elongation factor 2 kinase; Eukaryotic elongation factor 2 kinase; eukaryotic elongation factor-2 kinase; HSU93850; MGC45041\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 105\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46799448473769,"sku":"3691T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-3691t","provider":"Iright","version":"1.0","type":"link"}