{"product_id":"cst-3729s","title":"CST,  3729S, OSR1 Antibody","description":"Polyclonal Antibody for studying OSR1. Validated for Western Blotting. Highly specific and rigorously validated in-house, OSR1 Antibody (CST #3729) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nOSR1 Antibody detects endogenous levels of total OSR1 protein. This antibody does not cross-react with SPAK or other members of the GCK family.\nSpecies Reactivity: Human, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues at the amino terminus of human OSR1. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nSPAK (STE20\/SPS1-related Pro\/Ala-rich kinase) and OSR1 (oxidative stress responsive 1) are members of the GCK family of serine\/threonine kinases. Overexpression and studies demonstrate that SPAK is able to activate p38 MAP kinase, indicating a possible role for SPAK in the stress response (1). Yeast two-hybrid screening revealed that SPAK and OSR1 bind to Na-K-2Cl cotransporters NKCC1 and NKCC2 and K-Cl cotransporter KCC3 (2). WNK1 and WNK4 phosphorylate SPAK at Thr243\/247 and Ser380 (3-5). Similarly, WNK1 and WNK4 phosphorylate OSR1 at Thr185 and Ser315 (3,4). Phosphorylation at these sites stimulates SPAK and OSR1 activity, leading to NKCC1 phosphorylation and enhanced NKCC1 activity (3-5). SPAK is also phosphorylated at Ser311 by PKCθ in response to T cell activation. Substitution of Ser311 with Ala or specific siRNA knockdown of SPAK dramatically reduces TCR\/CD28-induced AP-1 activation, suggesting SPAK is involved in T cell signaling as well (6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nKIAA1101; OSR1; oxidative stress responsive 1; oxidative stress responsive kinase 1; Oxidative stress-responsive 1 protein; oxidative-stress responsive 1; OXSR1; Serine\/threonine-protein kinase OSR1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H Mk\nSENSITIVITY: Endogenous\nMW (kDa): 60\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46799451914409,"sku":"3729S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-3729s","provider":"Iright","version":"1.0","type":"link"}