{"product_id":"cst-37378t","title":"CST,  37378T, Cleaved N4BP1 (Asp488) (E8X4W) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying N4BP1 (Asp488, C-term) cleaved mouse. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Cleaved N4BP1 (Asp488) (E8X4W) Rabbit Monoclonal Antibody (CST #37378) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCleaved N4BP1 (Asp488) (E8X4W) Rabbit Monoclonal Antibody recognizes endogenous levels of N4BP1 protein only when cleaved at Asp488. A non-specific band has been observed at 62 kDa by Western Blot.\nSpecies Reactivity: Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp488 of mouse N4BP1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nN4BP1 (NEDD4-binding protein 1) was identified as a binding partner and substrate for the HECT domain-containing ubiquitin ligase NEDD4 (1). Subsequently, it was also found to interact with Itch, a ubiquitin ligase structurally similar to NEDD4 (2). Interaction between N4BP1 interferes with the ability of Itch to bind its substrates, including c-Jun and p63 (2). N4BP1 expression has been associated with immune regulation as well as cancer. N4BP1 was identified as an inhibitory factor of NF-κB-mediated MHC-1 expression in neuroblastoma and high expression of N4BP1 was linked to poor overall survival (3). Studies have also found that N4BP1 is a suppressor of cytokine production that can be cleaved and inactivated by caspase-8 (4). N4BP1 can interact with the NF-κB modulator NEMO to suppress NF-κB transcriptional activity (5). Studies have also identified N4BP1 as a potent interferon-inducible HIV-1 restriction factor that targets the degradation of viral mRNA (6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAI481586; C81621; Kiaa0615; MGC7607; N4BP1; NEDD4 binding protein 1; NEDD4-binding protein 1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: M\nSENSITIVITY: Endogenous\nMW (kDa): 70\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800091807913,"sku":"37378T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-37378t","provider":"Iright","version":"1.0","type":"link"}