{"product_id":"cst-3737s","title":"CST,  3737S, SUZ12 (D39F6) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying SUZ12. Validated for WB,IP,IF,F,ChIP,ChIP,C\u0026amp;R,eCLIP. Available in 2 sizes. Highly specific and rigorously validated in-house, SUZ12 (D39F6) Rabbit Monoclonal Antibody (CST #3737) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP and ChIP-seq results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT\u0026amp;RUN dilution was determined using CUT\u0026amp;RUN Assay Kit # 86652 .\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nImmunofluorescence (Immunocytochemistry): 1:800\nFlow Cytometry (Fixed\/Permeabilized): 1:100\nChromatin IP: 1:100\nChromatin IP-seq: 1:100\nCUT\u0026amp;RUN: 1:100\neCLIP: 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 53192 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized), Chromatin IP, Chromatin IP-seq, CUT\u0026amp;RUN\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nSUZ12 (D39F6) Rabbit Monoclonal Antibody detects endogenous levels of SUZ12 protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro257 of human Suz12 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe polycomb group (PcG) proteins contribute to the maintenance of cell identity, stem cell self-renewal, cell cycle regulation and oncogenesis by maintaining the silenced state of genes that promote cell lineage \u003cb\u003eSpecification\u003c\/b\u003e, cell death and cell-cycle arrest (1-4). PcG proteins exist in two complexes that cooperate to maintain long-term gene silencing through epigenetic chromatin modifications. The first complex, EED-EZH2, is recruited to genes by DNA-binding transcription factors and methylates histone H3 on Lys27. Methylation of Lys27 facilitates the recruitment of the second complex, PRC1, which ubiquitinylates histone H2A on Lys119 (5). Suppressor of Zeste 12 (SUZ12) is a component of the PRC2 complex, which together with Ezh2 and Eed is absolutely required for histone methyl-transferase activity (6). SUZ12 contains a C2H2 zinc finger domain similar to the zinc finger domains found in sequence-specific DNA binding proteins and may mediate the interaction between EZH2 and nucleosomes (6). SUZ12 is overexpressed in several human tumors, including tumors of the colon, breast and liver (7,8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nChET 9 protein; CHET9; Chromatin precipitated E2F target 9 protein; IMMAS; JJAZ1; Joined to JAZF1 protein; KIAA0160; Polycomb protein SUZ12; suppressor of zeste 12 homolog (Drosophila); Suppressor of zeste 12 protein homolog; SUZ12; SUZ12 polycomb repressive complex 2 subunit\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 83\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799452930217,"sku":"3737S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-3737s","provider":"Iright","version":"1.0","type":"link"}