{"product_id":"cst-3743s","title":"CST,  3743S, Rig-I (D14G6) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying RIG-I. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Rig-I (D14G6) Rabbit Monoclonal Antibody (CST #3743) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nRig-I (D14G6) Rabbit Monoclonal Antibody detects endogenous levels of total Rig-I protein.\nSpecies Reactivity: Human, Mouse, Rat, Hamster, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues Lys652 of human Rig-I.\n\u003cb\u003eBackground\u003c\/b\u003e\nAntiviral innate immunity depends on the combination of parallel pathways triggered by virus detecting proteins in the Toll-like receptor (TLR) family and RNA helicases, such as Rig-I (retinoic acid-inducible gene I) and MDA-5 (melanoma differentiation-associated antigen 5), which promote the transcription of type I interferons (IFN) and antiviral enzymes (1-3). TLRs and helicase proteins contain sites that recognize the molecular patterns of different virus types, including DNA, single-stranded RNA (ssRNA), double-stranded RNA (dsRNA), and glycoproteins. These antiviral proteins are found in different cell compartments; TLRs (i.e. TLR3, TLR7, TLR8, and TLR9) are expressed on endosomal membranes and helicases are localized to the cytoplasm. Rig-I expression is induced by retinoic acid, LPS, IFN, and viral infection (4,5). Both Rig-I and MDA-5 share a DExD\/H-box helicase domain that detects viral dsRNA and two amino-terminal caspase recruitment domains (CARD) that are required for triggering downstream signaling (4-7). Rig-I binds both dsRNA and viral ssRNA that contains a 5'-triphosphate end not seen in host RNA (8,9). Though structurally related, Rig-I and MDA-5 detect a distinct set of viruses (10,11). The CARD domain of the helicases, which is sufficient to generate signaling and IFN production, is recruited to the CARD domain of the MAVS\/VISA\/Cardif\/IPS-1 mitochondrial protein, which triggers activation of NF-κB, TBK1\/IKKε, and IRF-3\/IRF-7 (12-15).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAntiviral innate immune response receptor RIG-I; DDX58; DEAD (Asp-Glu-Ala-Asp) box polypeptide 58; DEAD box protein 58; DEAD\/H (Asp-Glu-Ala-Asp\/His) box polypeptide; DExD\/H-box helicase 58; DKFZp434J1111; DKFZp686N19181; FLJ13599; Probable ATP-dependent RNA helicase DDX58; retinoic acid inducible gene I; Retinoic acid-inducible gene 1 protein; Retinoic acid-inducible gene I protein; RIG-1; RIG-I; RIG-I-like receptor 1; RIG1; RIGI; RLR-1; RNA helicase RIG-I; SGMRT2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Hm Mk\nSENSITIVITY: Endogenous\nMW (kDa): 102\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799453782185,"sku":"3743S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-3743s","provider":"Iright","version":"1.0","type":"link"}