{"product_id":"cst-37481sf","title":"CST,  37481SF, TdTomato (E3G5L) Rabbit Monoclonal Antibody (BSA and Azide Free)","description":"Monoclonal Antibody for studying . Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, TdTomato (E3G5L) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #37481) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nThis product is the carrier free version of product #20163. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT\u0026amp;RUN or CUT\u0026amp;Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions\/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.\n\u003cb\u003eFormulation\u003c\/b\u003e\nSupplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 20163\n\u003cb\u003eStorage\u003c\/b\u003e\nStore at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze\/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nTdTomato (E3G5L) Rabbit Monoclonal Antibody (BSA and Azide Free) detects transfected and transgenic levels of TdTomato-tagged proteins (either N-terminally tagged or C-terminally tagged). Please note that the TdTomato tags add approximately 54.2 kDa to the molecular weight of the fusion protein.\nSpecies Reactivity: All Species Expected\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of TdTomato protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nEpitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. TdTomato is a fluorescent, photostable orange dye that was isolated from and further engineered from the Discosoma sea anemone (1). Reported to be six times brighter than EGFP, TdTomato is comprised of a tandem dimer of monomers engineered from DsRed; the dimers are held together by intramolecular bonds. For this reason, TdTomato is considered a monomer. Its peak fluorescent excitation is at 554 nm and emission is at 581 nm (1).\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: All\nSENSITIVITY: Transfected Only\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800216359081,"sku":"37481SF","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-37481sf","provider":"Iright","version":"1.0","type":"link"}