{"product_id":"cst-37489s","title":"CST,  37489S, MTA3 (E3X2E) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying MTA3. Validated for Western Blotting,Immunoprecipitation,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, MTA3 (E3X2E) Rabbit Monoclonal Antibody (CST #37489) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP results, use 5 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits.\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\nChromatin IP: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Chromatin IP\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nMTA3 (E3X2E) Rabbit Monoclonal Antibody recognizes endogenous levels of total MTA3 protein. This antibody does not recognize MTA1 or MTA2 protein. This antibody detects a 200 kDa protein of unknown origin.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val438 of human MTA3 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nMetastasis associated gene 3 (MTA3) is a cell-type specific subunit of the NuRD deacetylase complex. Much like MTA1 and MTA2, MTA3 does not associate with other family members, forming a unique NuRD complex. MTA3 has been shown to be estrogen-dependent and a key regulator of Snail and E-cadherin in breast cancer (1). B lymphocyte differentiation has been shown to be dependent on the ability of BCL6 to use MTA3 as a cofactor (2). MTA3 has also been shown to repress the Wnt4 pathway (3). MTA3 activity has been described in many different cancer types, including breast, non-small cell lung cancer, and B-cell lymphomas (1,4,5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nKIAA1266; metastasis associated 1 family member 3; metastasis associated 1 family, member 3; metastasis associated family, member 3; metastasis associated gene family, member 3; Metastasis-associated protein MTA3; MTA3\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 62, 66\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799454437545,"sku":"37489S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-37489s","provider":"Iright","version":"1.0","type":"link"}