{"product_id":"cst-37592t","title":"CST,  37592T, PR3 (F5M5D) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying PRTN3. Validated for Western Blotting,Flow Cytometry (Fixed\/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, PR3 (F5M5D) Rabbit Monoclonal Antibody (CST #37592) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nFlow Cytometry (Fixed\/Permeabilized): 1:100 - 1:400\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPR3 (F5M5D) Rabbit Monoclonal Antibody recognizes endogenous levels of total PR3 protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro145 of human PR3 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nProteinase 3 (PR3 or PRTN3), also known as myeloblastin, is a serine protease primarily expressed in neutrophils (1). PR3 can be localized to both azurophilic granules and the plasma membrane, where it serves as a target of antineutrophil cytoplasmic autoantibodies (ANCA) in the autoimmune condition granulomatosis with polyangiitis, formerly known as Wegener's granulomatosis (2). PR3 can degrade elastin, a component of the extracellular matrix, and activate platelets, dendritic cells, and endothelial cells by interacting with the proteinase-activated receptors (PARs), PAR-1 and PAR-2 (3-6). PR3 is also capable of processing inflammatory cytokines, including TNF-α, IL-1β, and IL-18 (7). PR3 has recently been shown to play a role in hematopoietic differentiation by participating in the cleavage of histone H3. Through its ability to modulate Stat3 to promote myeloid differentiation, it may serve as a therapeutic target in acute myeloid leukemia (7,8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nACPA; AGP7; azurophil granule protein 7; C-ANCA; C-ANCA antigen; CANCA; Leukocyte proteinase 3; MBN; MBT; Myeloblastin; Neutrophil proteinase 4; NP-4; NP4; P29; PR-3; PR3; proteinase 3; PRTN3; serine proteinase, neutrophil; Wegener autoantigen; Wegener granulomatosis autoantigen\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 27-40\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799455617193,"sku":"37592T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-37592t","provider":"Iright","version":"1.0","type":"link"}