{"product_id":"cst-37635t","title":"CST,  37635T, PYCR1 (E7P3I) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying PYCR1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, PYCR1 (E7P3I) Rabbit Monoclonal Antibody (CST #37635) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPYCR1 (E7P3I) Rabbit Monoclonal Antibody recognizes endogenous levels of total PYCR1 protein.\nSpecies Reactivity: Human, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala114 of human PYCR1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nMitochondrial pyrroline-5-carboxylate reductase 1 (PYCR1) catalyzes the last step in proline biosynthesis (1). Research studies show that proline is a limiting amino acid to clear cell renal cell carcinoma (ccRCC). expression is induced in kidney cancer cells to compensate for the proline shortage and maintain cancer cell growth. Furthermore, knockdown inhibits ccRCC cell proliferation in the absence of proline, suggesting this enzyme as a potential cancer therapeutic target (2). In addition, breast cancer cells also express high levels of PYCR1 (2,3). Proline is restrictive to invasive ductal breast carcinoma cells, indicating proline vulnerability in breast cancer formation (2).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nARCL2B; ARCL3B; P5C; P5C reductase 1; P5CR; P5CR 1; P5CR1; PIG45; PP222; PRO3; proliferation-inducing protein 45; PYCR; PYCR1; pyrroline-5-carboxylate reductase 1; Pyrroline-5-carboxylate reductase 1, mitochondrial\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H Mk\nSENSITIVITY: Endogenous\nMW (kDa): 30, 32\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799455813801,"sku":"37635T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-37635t","provider":"Iright","version":"1.0","type":"link"}