{"product_id":"cst-3806t","title":"CST,  3806T, Phospho-Rictor (Thr1135) (D30A3) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Rictor (Thr1135) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Rictor (Thr1135) (D30A3) Rabbit Monoclonal Antibody (CST #3806) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-Rictor (Thr1135) (D30A3) Rabbit Monoclonal Antibody detects endogenous levels of rictor protein only when phosphorylated at Thr1135.\nSpecies Reactivity: Human, Mouse, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to the sequence surrounding Thr1135 of human Rictor protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian TOR (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals (1). mTOR is a large protein kinase with two different complexes. One complex contains mTOR, GβL and raptor, which is a target of rapamycin. The other complex, insensitive to rapamycin, includes mTOR, GβL, Sin1, and rictor (1). The mTOR-rictor complex phosphorylates Ser473 of Akt\/PKB (2). This phosphorylation is essential for full Akt\/PKB activation. Furthermore, an siRNA knockdown of rictor inhibits Ser473 phosphorylation in 3T3-L1 adipocytes (3). This complex has also been shown to phosphorylate the rapamycin-resistant mutants of S6K1, another effector of mTOR (4). Phosphorylation of Thr1135 on rictor was identified at Cell Signaling Technology (CST) using PhosphoScan , CST's LC-MS\/MS platform for phosphorylation site discovery (5). Additional research indicates that rictor is phosphorylated at Thr1135 by p70 S6K, which negatively regulates mTORC2 protein complex as part of a negative feedback mechanism controlling Akt activity (6-8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAVO3; AVO3 homolog; DKFZp686B11164; hAVO3; KIAA1999; mAVO3; MGC39830; PIA; pianissimo; Rapamycin-insensitive companion of mTOR; RICTOR; RICTR; RPTOR independent companion of MTOR complex 2; RPTOR independent companion of MTOR, complex 2; TORC2-specific protein AVO3\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M Mk\nSENSITIVITY: Endogenous\nMW (kDa): 200\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799459319977,"sku":"3806T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-3806t","provider":"Iright","version":"1.0","type":"link"}