{"product_id":"cst-3822s","title":"CST,  3822S, MST4 Antibody","description":"Polyclonal Antibody for studying MST4. Validated for Western Blotting. Highly specific and rigorously validated in-house, MST4 Antibody (CST #3822) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nMST4 Antibody recognizes endogenous levels of total MST4 protein independent of phosphorylation. This antibody does not cross-react with MST1-3 proteins.\nSpecies Reactivity: Human, Mouse, Rat, Monkey, Bovine\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal residues of human MST4 protein. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nMammalian sterile-20-like (MST) kinases are upstream regulators of mitogen-activated protein kinase (MAPK) signaling pathways that regulate multiple biological processes, including apoptosis, morphogenesis, cell migration, and cytoskeletal rearrangements (1). This group of serine\/threonine kinases includes a pair of closely related proteins (MST1, MST2) that are functionally distinct from the more distantly related MST3 and MST4 kinases. All four MST kinases share a conserved amino-terminal kinase domain and carboxy-terminal regulatory and interaction domains (1-3). At least three of these kinases (MST1-3) promote apoptosis and are activated by caspase cleavage followed by nuclear translocation of the active kinase. MST1\/2 kinases play a key role in the Hippo signaling pathway, an evolutionarily conserved program that controls organ size by regulating cell proliferation, apoptosis, and stem cell self renewal (4). Mammalian Sterile 20-like kinase 4 (MST4, STK26, MASK) is a Golgi-localized kinase that is cleaved by caspase-3 . While its potential role in apoptosis is unclear, research studies indicate that MST4 is involved in MAPK and EGF pathway signaling (5,6). MST4 and the serine\/threonine kinase YSK1 (STK25) localize to the Golgi apparatus following association with the Golgi scaffold protein GM130. Binding to GM130 activates MST4 through autophosphorylation at Thr178 (7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nMammalian STE20-like protein kinase 4; mammalian sterile 20-like 4; MASK; MST-4; Mst3 and SOK1-related kinase; MST4; serine\/threonine kinase 26; serine\/threonine protein kinase 26; serine\/threonine protein kinase MASK; serine\/threonine protein kinase MST4; Serine\/threonine-protein kinase 26; Serine\/threonine-protein kinase MASK; Serine\/threonine-protein kinase MST4; STE20-like kinase 4; STE20-like kinase MST4; STK26\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk B\nSENSITIVITY: Endogenous\nMW (kDa): 52\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46799460696233,"sku":"3822S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-3822s","provider":"Iright","version":"1.0","type":"link"}