{"product_id":"cst-3833s","title":"CST,  3833S, eIF6 (D16E9) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying eIF6. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, eIF6 (D16E9) Rabbit Monoclonal Antibody (CST #3833) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nImmunofluorescence (Immunocytochemistry): 1:600\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\neIF6 (D16E9) Rabbit Monoclonal Antibody detects endogenous levels of total eIF6 protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human eIF6 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nEukaryotic initiation factor 6 (eIF6) is reqiured for the 60S ribosomal subunit assembly in the nucleolus (1). In the cytoplasm, this protein is bound to 60S ribosome subunits and prevents them from joining 40S ribosome subunits to form 80S ribosomes (2). eIF6 is also shown to associate with the RNA-induced silencing complex (RISC) (3). Deletion of eIF6 abolishes the miRNA-mediated gene silencing (3). eIF6 may play its essential role in miRNA-mediated silencing by inhibiting translation initiation or ribosome recycling (3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n2; b; b(2)gcn; B(2)GCN homolog; B4 integrin interactor; CAB; eIF-6; EIF3A; EIF6; eukaryotic translation initiation factor 3A; Eukaryotic translation initiation factor 6; gcn; IF6; integrin beta 4 binding protein; ITGB4BP; p27 beta-4 integrin-binding protein; p27(BBP); p27BBP\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 27\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799461810345,"sku":"3833S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-3833s","provider":"Iright","version":"1.0","type":"link"}