{"product_id":"cst-3842s","title":"CST,  3842S, LIMK1 Antibody","description":"Polyclonal Antibody for studying LIMK1. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, LIMK1 Antibody (CST #3842) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nLIMK1 Antibody detects endogenous levels of total LIMK1 protein independent of phosphorylation. This antibody does not cross-react with LIMK2.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminal residues of human LIMK1. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nLIM kinases (LIMK1 and LIMK2) are serine\/threonine kinases that have two zinc finger motifs, known as LIM motifs, in their amino-terminal regulatory domains (1). LIM kinases are involved in actin cytoskeletal regulation downstream of Rho-family GTPases, PAKs, and ROCK (2,3). PAK1 and ROCK phosphorylate LIMK1 or LIMK2 at the conserved Thr508 or Thr505 residues in the activation loop, increasing LIMK activity (3-5). Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the amino-terminal Ser3 residue of cofilin (6,7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nLIM domain kinase 1; LIM motif-containing protein kinase; LIMK; LIMK-1; LIMK1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 70\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46799462465705,"sku":"3842S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-3842s","provider":"Iright","version":"1.0","type":"link"}