{"product_id":"cst-3845s","title":"CST,  3845S, LIMK2 (8C11) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying LIMK2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, LIMK2 (8C11) Rabbit Monoclonal Antibody (CST #3845) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nLIMK2 (8C11) Rabbit Monoclonal Antibody detects endogenous levels of total LIMK2 protein. This antibody does not cross-react with LIMK1.\nSpecies Reactivity: Human, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to carboxy-terminal residues of human LIMK2.\n\u003cb\u003eBackground\u003c\/b\u003e\nLIM kinases (LIMK1 and LIMK2) are serine\/threonine kinases that have two zinc finger motifs, known as LIM motifs, in their amino-terminal regulatory domains (1). LIM kinases are involved in actin cytoskeletal regulation downstream of Rho-family GTPases, PAKs, and ROCK (2,3). PAK1 and ROCK phosphorylate LIMK1 or LIMK2 at the conserved Thr508 or Thr505 residues in the activation loop, increasing LIMK activity (3-5). Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the amino-terminal Ser3 residue of cofilin (6,7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nLIM domain kinase 2; LIMK-2; LIMK2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H Mk\nSENSITIVITY: Endogenous\nMW (kDa): 70\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799462891689,"sku":"3845S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-3845s","provider":"Iright","version":"1.0","type":"link"}