{"product_id":"cst-38470s","title":"CST,  38470S, G4S Linker (E7O2V) Rabbit Monoclonal Antibody (InTraSeq™ 3' Conjugate 3118)","description":"Monoclonal Antibody for studying . Validated for Single Cell Analysis - InTraSeq™ 3'. Highly specific and rigorously validated in-house, G4S Linker (E7O2V) Rabbit Monoclonal Antibody (InTraSeq\u003csup\u003e™\u003c\/sup\u003e 3' Conjugate 3118) (CST #38470) is ready to ship.\n\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in PBS (pH 7.2), 2 mM EDTA, 0.05% Triton X-100, 2 mg\/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Single Cell Analysis - InTraSeq™ 3'\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nG4S Linker (E7O2V) Rabbit Monoclonal Antibody (InTraSeq™ 3' Conjugate 3118) recognizes exogenously expressed levels of scFv-based CARs containing a G4S linker.\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide containing three Gly4Ser repeats.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe poly-Glycine-Serine (G4S) linker is a type of flexible, unstructured synthetic peptide linker sequence often leveraged to connect the variable heavy (VH) domain and variable light (VL) domain of single-chain variable fragments (scFvs) and chimeric antigen receptors (CARs) that utilize an extracellular domain scFv for target antigen recognition. The linker itself consists of a core pentapeptide sequence, Gly-Gly-Gly-Gly-Ser, that is repeated and commonly found as either a 15-mer (G4S) or 20-mer (G4S) within scFv-based CARs and scFv fragments. The linker sequence length plays a role in controlling scFv stability and the noncovalent association between the VH and VL domains (1,2).\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Transfected Only\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800390127785,"sku":"38470S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-38470s","provider":"Iright","version":"1.0","type":"link"}