{"product_id":"cst-38548s","title":"CST,  38548S, Whitlow\/218 Linker (E3U7Q) Human Chimeric Monoclonal Antibody","description":"Monoclonal Antibody for studying . Validated for Flow Cytometry (Live). Available in 2 sizes. Highly specific and rigorously validated in-house, Whitlow\/218 Linker (E3U7Q) Human Chimeric Monoclonal Antibody (CST #38548) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFlow Cytometry (Live): 1:50 - 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Flow Cytometry (Live)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nWhitlow\/218 Linker (E3U7Q) Human Chimeric mAb recognizes exogenously expressed levels of scFv-based CARs containing a Whitlow\/218 linker. This antibody is not approved for traditional Western blot analysis.\nSpecies Reactivity: All Species Expected\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nThis recombinant chimeric antibody is engineered from Whitlow\/218 Linker (E3U7Q) Rabbit mAb #57710 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody but contains a human-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies. The parent antibody, Whitlow\/218 (E3U7Q) Rabbit mAb #57710, is produced by immunizing animals with a synthetic peptide corresponding to residues of the Whitlow\/218 linker peptide.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe Whitlow\/218 peptide is a commonly used linker that connects the variable heavy (VH) domain and variable light (VL) domain of single-chain variable fragments (scFvs) and chimeric antigen receptors (CARs) that utilize an extracellular domain scFv for target antigen recognition. The Whitlow\/218 linker affords the scFv with enhanced resistance to proteolysis and aggregation (1).\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: All\nSENSITIVITY: Transfected Only\nSource\/Isotype: Human IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800330883241,"sku":"38548S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-38548s","provider":"Iright","version":"1.0","type":"link"}