{"product_id":"cst-3856s","title":"CST,  3856S, ILK1 (4G9) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying ILK1. Validated for Western Blotting,Immunoprecipitation,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, ILK1 (4G9) Rabbit Monoclonal Antibody (CST #3856) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nImmunohistochemistry (Paraffin): 1:100 - 1:400\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 53303 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nILK1 (4G9) Rabbit Monoclonal Antibody detects endogenous levels of total ILK1 protein. This antibody may cross-react with ILK2.\nSpecies Reactivity: Human, Mouse, Rat, Hamster, Monkey, Zebrafish, Bovine, Pig\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human ILK1.\n\u003cb\u003eBackground\u003c\/b\u003e\nIntegrin-linked kinases (ILKs) couple integrins and growth factors to downstream pathways involved in cell survival, cell cycle control, cell-cell adhesion and cell motility (1). ILK functions as a scaffold bridging the extracellular matrix (ECM) and growth factor receptors to the actin cytoskeleton through interactions with integrin, PINCH (which links ILK to the RTKs via Nck2), CH-ILKBP and affixin (1). ILK phosphorylates Akt at Ser473, GSK-3 on Ser9, myosin light chain 2 (MLC2) on Ser18\/Thr19, as well as affixin (2-5). These phosphorylation events are key regulatory steps in modulating the activities of the targets. ILK activity is stimulated by PI3 kinase and negatively regulated by the tumor suppressor PTEN and a PP2C protein phosphatase, ILKAP (1,3,6). It has been suggested that the conserved Ser343 residue in the activation loop plays a key role in the activation of ILK1 (2).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n59 kDa serine\/threonine-protein kinase; DKFZp686F1765; epididymis secretory protein Li 28; HEL-S-28; ILK; ILK-1; ILK-2; ILK1; ILK2; integrin linked kinase; integrin-linked kinase; integrin-linked kinase-2; Integrin-linked protein kinase; P59; p59ILK\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Hm Mk Z B Pg\nSENSITIVITY: Endogenous\nMW (kDa): 51\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799463710889,"sku":"3856S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-3856s","provider":"Iright","version":"1.0","type":"link"}