{"product_id":"cst-3867s","title":"CST,  3867S, nSMase1 Antibody","description":"Polyclonal Antibody for studying SMPD2. Validated for Western Blotting. Highly specific and rigorously validated in-house, nSMase1 Antibody (CST #3867) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nnSMase1 Antibody detects endogenous levels of total nSMase1 protein.\nSpecies Reactivity: Human, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala200 of human nSMase1.\n\u003cb\u003eBackground\u003c\/b\u003e\nSphingomyelinases (SMases) catalyze sphingomyelin hydrolysis to produce ceramide and phosphocholine (1). Ceramide is an important bioactive lipid triggering signal transduction in cell proliferation, apoptosis, and differentiation (1,2). Many SMases have been described and categorized based on their optimum pH activity, cation dependence, tissue distribution, and subcellular localization (1). These include a lysosomal acid SMase, a Zn -dependent secreted acid SMase, a membrane-bound Mg -dependent neutral SMase, a Mg -independent neutral SMase, and an alkaline SMase. nSMase1 (also termed SMPD2) is a Mg -dependent neutral SMase that is widely expressed and predominantly localized to the endoplasmic reticulum (3,4). This protein has also been shown to have lyso-platelet activating factor (PAF) phospholipase C activity (5). A second neutral SMase, nSMase2 (also termed SMPD3) is predominantly expressed in the brain (6). The activity of neutral SMases is regulated by oxidative stress, chemotherapeutic drugs, inflammatory cytokines, and apoptotic stimuli (1). Analysis of single and double knockouts of the SMPD2 and SMPD3 has revealed that loss of both genes leads to complete loss of neutral SMase activity with developmental defects observed with loss of nSMase2 (7,8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nISC1; Lyso-PAF-PLC; Lyso-platelet-activating factor-phospholipase C; N-SMase; Neutral sphingomyelinase; NSMA; nSMase; NSMASE1; SMPD2; Sphingomyelin phosphodiesterase 2; sphingomyelin phosphodiesterase 2, neutral membrane (neutral sphingomyelinase)\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H Mk\nSENSITIVITY: Endogenous\nMW (kDa): 50\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46799464530089,"sku":"3867S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-3867s","provider":"Iright","version":"1.0","type":"link"}