{"product_id":"cst-38774s","title":"CST,  38774S, CD28 (D2Z4E) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying CD28. Validated for WB,IP,IHC,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, CD28 (D2Z4E) Rabbit Monoclonal Antibody (CST #38774) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nIHC Leica Bond: 1:400 - 1:1600\nImmunohistochemistry (Paraffin): 1:50 - 1:200\nImmunofluorescence (Frozen): 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 87823 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, IHC Leica Bond, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCD28 (D2Z4E) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD28 protein and is predicted to react with multiple CD28 isoforms that are produced through alternative splicing. This antibody cross-reacts with 16 kDa and 100 kDa proteins of unknown origin in some mouse cells. Non-specific staining was observed in mouse testis by immunohistochemistry.\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro208 of human CD28 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCD28 is a transmembrane glycoprotein expressed by T cells as well as some other hematopoietic cells (1,2). T cell activation requires T cell receptor (TCR) recognition of antigen presented in the context of MHC molecules. CD28 acts as a T cell costimulatory receptor, and interaction of CD28 with its ligands CD80 or CD86 provides the second signal required for naïve T cell activation (3-5). Activation of naïve T cells in the absence of CD28 stimulation can result in a state of T cell anergy, or unresponsiveness (3). CD28 signals through cytoplasmic phospho-tyrosine motifs that bind several SH2 or SH3 domain-containing proteins involved in T cell activation (2). Recently, CD28 was demonstrated to be a preferred target of PD-1-mediated dephosphorylation. Consistently, CD28 expression was required for T cell proliferation following PD-1 blockade and CD28 stimulation was required for effective anti-PD-1 cancer immunotherapy in mice (6,7). Several CD28 isoforms are produced by alternative splicing (8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCD28; CD28 antigen; CD28 molecule; MGC138290; T-cell-specific surface glycoprotein CD28; TP44\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nMW (kDa): 14, 40-60\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799465971881,"sku":"38774S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-38774s","provider":"Iright","version":"1.0","type":"link"}