{"product_id":"cst-38889s","title":"CST,  38889S, S6 Ribosomal Protein (54D2) Mouse Monoclonal Antibody (InTraSeq™ 3' Conjugate 3005)","description":"Monoclonal Antibody for studying S6 Ribosomal Protein. Validated for Single Cell Analysis - InTraSeq™ 3'. Highly specific and rigorously validated in-house, S6 Ribosomal Protein (54D2) Mouse Monoclonal Antibody (InTraSeq\u003csup\u003e™\u003c\/sup\u003e 3' Conjugate 3005) (CST #38889) is ready to ship.\n\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in PBS (pH 7.2), 2 mM EDTA, 0.05% Triton X-100, 2 mg\/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Single Cell Analysis - InTraSeq™ 3'\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nS6 Ribosomal Protein (54D2) Mouse Monoclonal Antibody (InTraSeq™ 3' Conjugate 3005) detects endogenous levels of total S6 ribosomal protein independent of phosphorylation.\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a recombinant fusion protein corresponding to full-length human S6 ribosomal protein. The epitope corresponds to a region surrounding Asp20 of human S6 ribosomal protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nOne way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions (2). These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression, as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of S6 protein (4,5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n40S ribosomal protein S6; Phosphoprotein NP33; ribosomal protein S6; RPS6; RS6; S6; S6 ribosomal protein; Small ribosomal subunit protein eS6\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nSource\/Isotype: Mouse IgG1","brand":"CST","offers":[{"title":"Default Title","offer_id":46800777019561,"sku":"38889S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-38889s","provider":"Iright","version":"1.0","type":"link"}