{"product_id":"cst-38910s","title":"CST,  38910S, TRIM16 (F3S3I) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying TRIM16. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, TRIM16 (F3S3I) Rabbit Monoclonal Antibody (CST #38910) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nImmunofluorescence (Immunocytochemistry): 1:800\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nTRIM16 (F3S3I) Rabbit mAb recognizes endogenous levels of total TRIM16 protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala69 of human TRIM16 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nMisfolded proteins are cleared in cells by ubiquitination and degradation using the proteasome and autophagy pathways (1-5). Various factors, including mutations, oxidative stress, and environmental conditions, can trigger increased protein misfolding and overwhelm cells, resulting in aggregation, accumulation, and disease (2,3). Tripartite Motif-Containing Protein 16 (TRIM16) was first discovered to regulate the NRF2 pathway (3) along with other autophagy proteins, such as Beclin-1, ULK1, and ATG16L, helping to facilitate protein aggregate clearing (1-3,6). TRIM16 plays an important role in cellular defense against oxidative stress and autophagy (4-6). The TRIM family of proteins is highly conserved and has been implicated in a diverse range of biological processes, including development, cell growth, differentiation, innate immune functions, and cancer (4). TRIMs most commonly have a RING finger domain, B-box domains, a coiled-coil domain, and a variable C-terminal domain (4). Specifically, TRIM16 lacks a RING domain but possesses two B-box domains and a C-terminal SPRY domain. TRIM16 has recently been shown to function as an E3 ubiquitin ligase (1,4). TRIM16 is also studied as a tumor suppressor, influencing cell differentiation and migration, and has been implicated in various cancers (7-9), including pancreatic, bladder (9), and breast cancer (7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nE3 ubiquitin-protein ligase TRIM16; EBBP; Estrogen-responsive B box protein; TRI16; TRIM16; tripartite motif containing 16; tripartite motif-containing 16; Tripartite motif-containing protein 16\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 70\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799467085993,"sku":"38910S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-38910s","provider":"Iright","version":"1.0","type":"link"}