{"product_id":"cst-3911s","title":"CST,  3911S, Phospho-p56Dok-2 (Tyr351) Antibody","description":"Polyclonal Antibody for studying Dok2 (Tyr345) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-p56Dok-2 (Tyr351) Antibody (CST #3911) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-p56Dok-2 (Tyr351) Antibody detects transfected levels of p56Dok-2 only when phosphorylated at tyrosine 351. This antibody does not cross-react with other tyrosine phosphorylated p62Dok family members.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr351 of mouse p56Dok-2. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nDocking proteins are substrates of tyrosine kinases that function in the recruitment and assembly of specific signal transduction molecules. There are five members in the p62dok family, p62Dok (Dok-1), p56Dok-2 (Dok-2, or DoK-R), Dok-3, Dok-4 and Dok-5 (1-3), characterized by the presence of an amino-terminal PH domain, a central PTB domain and numerous potential sites of tyrosine phosphorylation. Tyrosine phosphorylation of p56Dok-2 occurs upon stimulation of cells with a variety of stimuli, or in cells transformed by oncogenic tyrosine kinases such as v-Src and Bcr-Abl (3-5). Based on the presence of several signaling domains (PH, PTB domain, tyrosine residue and proline-rich regions), it has been proposed that the p62dok family act as docking proteins that link RTKs to signal transduction pathways. p56Dok-2 has been proposed to be a negative regulator of cytokine-induced proliferation in T cells (5). Phosphorylated Tyr351 of p56Dok-2 mediates an association with the SH2 domain of Nck (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nDocking protein 2; docking protein 2, 56kD; docking protein 2, 56kDa; DOK2; Downstream of tyrosine kinase 2; p56(dok-2); p56DOK; p56dok-2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Transfected Only\nMW (kDa): 56 to 58\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46799468331177,"sku":"3911S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-3911s","provider":"Iright","version":"1.0","type":"link"}