{"product_id":"cst-3913t","title":"CST,  3913T, Tropomyosin-1\/3 (D17B8) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying TPM1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Tropomyosin-1\/3 (D17B8) Rabbit Monoclonal Antibody (CST #3913) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nTropomyosin-1\/3 (D17B8) Rabbit Monoclonal Antibody detects endogenous levels of total tropomyosin-1 and\/or tropomyosin-3 protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu274 of human tropomyosin-1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nTropomyosin-1 (TPM1) belongs to the high molecular weight members of tropomyosin family (1,2). The protein exists in an alpha-helical coiled-coil conformation and binds multiple acting monomers in a tight manner to stabilize and regulate the actin filament (3). Tropomyosins fullfill functions in muscle and non-muscle cells. In muscle cells, tropomyosins associate with the troponin complex and play a central role in the calcium-dependent regulation of striated muscle contraction in vertebrates. In non-muscle cells, tropomyosins are implicated in the formation and stabilization of cytoskeletal actin filaments to ensure normal cellular processes (1,2). Mutations of tropomysin-1 have been reported as a cause of dilated cardiac myopathies (4). Tropomyosin-1 also functions as a tumor suppressor, and many malignant tumors demonstrate downregulation of tropomyosin-1 expression (5-8). Tropomyosin-1 is phosphorylated at Ser283 through the Erk\/DAPK pathway, which promotes stress fiber formation in response to oxidative stress (9-10).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nalpha tropomyosin; Alpha-tropomyosin; C15orf13; cardiomyopathy, hypertrophic 3; CMD1Y; CMH3; epididymis secretory protein Li 265; HEL-S-265; HTM-alpha; LVNC9; sarcomeric tropomyosin kappa; TMSA; TPM1; tropomyosin 1; tropomyosin 1 (alpha); Tropomyosin alpha-1 chain; Tropomyosin-1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 32 , 35, 36, 41\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799468462249,"sku":"3913T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-3913t","provider":"Iright","version":"1.0","type":"link"}