{"product_id":"cst-3924t","title":"CST,  3924T, NSF (D31C7) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying NSF. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, NSF (D31C7) Rabbit Monoclonal Antibody (CST #3924) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\nImmunofluorescence (Frozen): 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 62908 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nNSF (D31C7) Rabbit Monoclonal Antibody detects endogenous levels of total NSF protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu524 of human NSF protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nSeveral protein-protein interactions are essential to membrane fusion during endocytosis. Membrane fusion requires interaction among SNARE1 proteins associated with both donor and acceptor membranes (1,2). Following membrane fusion, the α-SNAP cytoplasmic adapter protein binds to the SNARE complex. N-ethylmaleimide-sensitive factor (NSF), a hexameric ATPase, then associates with the α-SNAP\/SNARE complex to mediate SNARE disassembly during membrane fusion (3,4). The ATPase activity of NSF induces a conformational change in the α-SNAP\/SNARE complex that leads to its dissociation from the membrane, membrane fusion and eventual recycling of the SNARE complex for subsequent membrane fusion (3,4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nepididymis secretory sperm binding protein; N-ethylmaleimide sensitive factor, vesicle fusing ATPase; N-ethylmaleimide-sensitive factor; N-ethylmaleimide-sensitive factor-like protein; N-ethylmaleimide-sensitive fusion protein; NEM-sensitive fusion protein; NSF; SEC18; SKD2; Vesicle-fusing ATPase; Vesicular-fusion protein NSF\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 78\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799469183145,"sku":"3924T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-3924t","provider":"Iright","version":"1.0","type":"link"}